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Extra resources for Analyses for hormonal substances in food producing animals

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The study of Chakravarti et al. (2001)56 is cited as evidence that oestradiol3,4-quinone induces mutations in mouse skin in vivo. htm, accessed 29 March 2009. 26 Chapter 1 mutations in the H-ras gene. The dose used was 200 nmol oestradiol-3,4-quinone; the treated surface area was not defined. The mutations induced were sequenced. N3-adenine adducts (rapidly depurinating) and N7 guanine adducts (slowly depurinating) were seen. It appears that those arising at N3, but not at N7 guanine, gave rise to mutations.

From a starting concentration of 20 pg mlÀ1, falling to baseline by 24 hours. Thus, the increment in oestradiol from ingesting 2 mg was 90 pg mlÀ1¼320 pmol lÀ1. Over 24 hours the mean increment would be no more than 150 pmol lÀ1. 5th percentile of consumers eat 40 g of kidney per day). This increment is approximately three orders of magnitude below the most sensitive assays available and below any concerns related to breast cancer risk. 01%. 2 Reduced Breast Cancer Risk in Future The WG noted that in the UK in 2002, there were over 100,000 women receiving tamoxifen for treatment of breast cancer, of whom about 75% were postmenopausal.

25 for each doubling of plasma 17b-oestradiol concentrations. It is, however, near certain that this underestimates the true risk,vii since only a single blood sample from each subject was analysed in each of the studies. Additionally, the studies used a wide variety of analytical techniques, some of which were inaccurate and/or inappropriate for use in postmenopausal women. 29 Notably, this study also reported that the breast cancers presented at a significantly later stage in the HRT users. 28 Consistent with the WHI study, a higher incidence of breast cancer was found in combination HRT users.

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