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By Paul L. Munson, John Glover, Egon Diczfalusy and Robert E. Olson (Eds.)

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111 . . . 112 References . . . . . . . . . . , . . . . . . . . . . . . I. INTRODUCTION Progress in steroid analysis has been remarkable in the last 15 years. Much of our present knowledge in steroid endocrinology could not have been acquired without the development of radioimmunoassay and other competitive protein-binding methods. These methods have provided the sensitivity and speed required for measurement of physiological concentrations of steroid hormones in large series of samples (see Gray and James, 1979).

1980). , not as phenolate anions. Also, in other respects, extraction with Lipidex 1000 shows analogies with a solvent extraction. , 1974). Conjugated steroids, because of their water solubility, are poorly extracted by Lipidex 1000. Studies of the extraction of conjugated bile acids have shown that addition of decyltrimethylammonium bromide to the aqueous solution results in formation of ion pairs that are extracted by Lipidex 1000 (Dyfverman and Sjovall, 1979). It is possible that this principle could be used for charged steroid conjugates (see also Section II,A,2).

Slow reacting substance of anaphylaxis. Adu. Zmmunol. 10, 105- 144. 30 B. SAMUElSSON AND S. HAMMARSTROM Orange, R. , and Moore, E. G. (1976). The effect of thiols on the immunologic release of slow reacting substance of anaphylaxis: 11. Other i n vitro and i n viuo models. J . Immunol. 116, 392-397. Orange, R. , Murphy, R. , Karnovsky, M. , and Austen, K. F. (1973). The physicochemical characteristics and purification of slow reacting substance of anaphylaxis. J . Immunol. 110, 760-770. , and Hammarstrom, S.

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