Download Advances in Applied Microbiology, Vol. 3 by Wayne W Umbreit PDF

By Wayne W Umbreit

Meant for researchers in utilized microbiology and environmental engineers, this e-book covers such themes as environmental review of biotechnological approaches and microbial changes of haloaromatic and haloaliphatic compounds.

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Desiccansc 20°C. POOC. - rvr RT Papain digest broth 0 2-3 Months 24-30 Months 96 49-109 100 68 36 References Annear (1958a) Fry and Greaves (1951) 8'2 Weeks 20 Scott (1958) 2 Days 3 Months 0 Months 12 Months 17 16 15 14 Birkhaug (1951) 4°C. 0 6 Months 37 12 B@eand Evjen (1952) 5°C. 5°C. RT RT 3-4 Months 6 Months 1 Month 5 Months 100 5°C. 37°C. 37°C. 5°C. 37°C. 37°C. 0 12 Months 2 Months 12 Months 0 12 Months 2 Months 12 Months 10 3 1 % Sodium glutamate 1 % Sucrose 5°C. 37°C. 5°C. 37°C. 6 Months 6 Months 6 Months 6 Months 10% Sucrose and 1% gelatin - 0 Mycobacterium tuberculosis (BCG) 50% Glucose Milk whey plus 3 % glucose 50 % Glucose 1% Sucrose 1 % Sodium glutamate 25°C.

Also, in the presence of colloids or other protective substances the optimal rate of cooling is altered. Meryman has indicated that either glycerol, lactose, or glucose protects the cells by inhibiting the freezing of some water and thus excessive concentration of electrolytes is prevented. Lovelock and Bishop (1959) and Luyet and Keane (1952) presented evidence that protection afforded erythrocytes is due to penetration of protective substances. Lovelock and Bishop believed that dimethylsulfoxide protected erythrocytes more than did glycerol because the cells were more permeable to dimethylsulfoxide than to glycerol.

1960) Hornibrook (1950) - Hutton et al. (1951) Rhoades (1958) 26 Weeks 4gL van Drimmelen and Steyn (1958) 50 45 17 Verwey (1945) Rr 0 19 Months 19 Months RT 3 Years - 0 20 Days 84 Days 84 Days 56 Days 84 Days __ 3°C. 3°C. 3°C. 3°C. 3°C. 00015 100 Heller (1941) 50 33 PRESERVATION OF BACTERIA BY LYOPHILIZATION TABLE I (Continued) Storage Organism and suspending fluid Temperature Time 5 % Lactose plus salts Mist,. desiccansc - - RT RT Serum Peptone Serum Skim milk Neisseria 10% Glucose and 10% peptone on starch 0 0 8-14 Months 24-30 Months RT 10 Years - 0 0 0 40 Days 10 Days - 10°C.

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